Expression développementale de dlx5a et chm chez le poisson-zèbre

Auteurs-es

  • Maïka Harvey University of Ottawa, Ottawa, ON, Canada
  • Ben Harrison University of Ottawa, Ottawa, ON, Canada
  • Kathleen M. Gilmour University of Ottawa, Ottawa, ON, Canada
  • Marc Ekker University of Ottawa, Ottawa, ON, Canada

DOI :

https://doi.org/10.18192/osurj.v5i2.8052

Résumé

Les larves de poisson-zèbre (Danio rerio) constituent un modèle puissant pour visualiser l’expression génique précoce chez les vertébrés en raison de leur développement externe et de la conservation de différentes séquences géniques avec leurs homologues humains, ce qui leur permet d’être des outils utiles pour les comparaisons entre espèces. Le gène dlx5a code un facteur de transcription à homéodomaine exprimé dans les régions craniofaciales et dérivées de la crête neurale, tandis que chm code la protéine d’escorte Rab 1 (REP-1), qui est largement requise pour le trafic intracellulaire et le développement rétinien. Nous avons cherché à caractériser l’expression développementale de base de dlx5a et de chm dans des embryons de poisson-zèbre de type sauvage à 2 et 3 jours post-fécondation (jpf). Une hybridation in situ sur embryon entier a été réalisée sur des embryons fixés au paraformaldéhyde et conservés dans le méthanol à l’aide de sondes antisens marquées à la digoxigénine pour dlx5a ou chm, respectivement. À 2 jpf, le marquage de dlx5a a été détecté dans des domaines distincts de la tête antérieure et le long de l’axe du corps, ce qui est cohérent avec les régions de patterning craniofaciales et liées à la crête neurale. À 3 jpf, le signal de dlx5a semblait plus fort et plus étendu dans ces domaines, reflétant le développement continu des structures positives pour dlx5a. En revanche, le marquage de chm montrait un changement développemental de localisation. À 2 jpf, l’expression était principalement restreinte à la région de la tête. À 3 jpf, le signal de chm devenait plus apparent dans le tronc, indiquant une expansion de la distribution du transcrit le long de l’axe du corps, ce qui est cohérent avec son patron d’expression ubiquitaire et essentiel chez le poisson-zèbre, la souris et l’humain. Ensemble, ces cartes qualitatives d’expression établissent une base développementale pour de futures études examinant comment des mutations dans chm ou dans dlx5a, ou des perturbations environnementales, modifient la localisation génique dans des modèles pertinents de poisson-zèbre.

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Publié-e

2026-06-17

Numéro

Vol. 5 No. 2 (2026)

Rubrique

Résumés d’initiation à la recherche en sciences au premier cycle

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