Titre: Investigation de l'interférence ARN médiée par la souche HT115 d’E. coli dans le traitement de l’infection gastro-intestinale nématode Ascaris lumbricoides
DOI :
https://doi.org/10.18192/osurj.v4i1.7421Résumé
Les nématodes sont des vers parasites présents dans le corps qui peuvent entraîner divers problèmes de santé. Un sous-groupe de ces nématodes s’appelle Ascaris lumbricoides, qui prolifèrent dans l’intestin. Il s’agit du ver parasite le plus courant chez l’humain, infectant des centaines de millions de personnes mondialement. Un mode courant d’infection par les nématodes est le contact avec un sol contaminé par leurs œufs. Cette expérience tente de perturber le gène VENOM du A. lumbricoides, afin d’empêcher son fonctionnement chez l’hôte humain et de provoquer l’expulsion du ver. L’expérience commence par la modification d’une bactérie intestinale pour qu’elle délivre des molécules d’interférence ARN ciblant le gène VENOM, entraînant ainsi l’expulsion du parasite. Nous proposons deux méthodes de livraison pour la sécrétion: la première consiste à utiliser des vésicules de la membrane externe pour emballer et transporter les molécules d’interférence ARN et la seconde consiste à sécréter directement l’ARNi à l’aide d’un système de sécrétion bactérien de type 3. Nous supposons que dans les deux méthodes, après la livraison de l’ARNi, celui-ci sera absorbé par le ver et régulera négativement le gène VENOM, menant à la mort du ver. Ces résultats pourraient offrir des informations précieuses pour améliorer les thérapies actuelles et potentiellement fournir des pistes sur la manière de prévenir la récurrence des infections par les nématodes.
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