Derivatization of Rosemary is Integral to its Analysis

Ahona Deb; Sharon Barden; Paul Mayer

doi:10.18192/osurj.v5i1.7566

La dérivatisation du romarin est intégrale à son analyse.

Auteurs-es

Ahona Deb University of Ottawa, Ottawa, ON, Canada
Sharon Barden University of Ottawa, Ottawa, ON, Canada
Paul Mayer University of Ottawa, Ottawa, ON, Canada

DOI :

https://doi.org/10.18192/osurj.v5i1.7566

Résumé

Les plantes de la famille des Lamiacées, tel le romarin (Salvia rosmarinus), la sauge commune (Salvia officinalis) et la sauge blanche (Salvia apiana) sont connues pour leurs saveurs prononcées, leurs feuilles parfumées et leurs sources riches de composés bioactifs. Une caractéristique clé partagée par ces plantes est leur teneur élevée en acide carnosique, un diterpène phénolique important, ainsi que ses dérivées. Cependant, l’analyse de ces composés via la chromatographie en phase gazeuse couplée à la spectrométrie de masse (CPG-SM) pose un défi et n’est pas fréquemment explorée dû à leur polarité élevée et leur faible volatilité.

Les techniques de dérivatisation telles la triméthylsilylation (TMS) et la sélection de solvant approprié peuvent remédier à ces limitations en améliorant la volatilité des composés, ainsi permettant à une identification par CPG-SM efficace par des modèles de fragmentation caractéristiques. Nous avons utilisé la dérivatisation triméthylsilylée d'extraits d'acétonitrile de romarin, de sauge blanche et de sauge commune, et avons quantifié l'acide carnosique, le rosmanol et l'acide 12-O-méthylcarnosique. Le romarin contenait le montant le plus élevé d’acide carnosique à 3,4 mg/mL, suivi par la sauge commune à 2,0 mg/mL et la sauge blanche à 0,7 mg/mL. Le romarin contenait le plus de rosmanol à 2,2 mg/mL suivi par la sauge commune (0,3 mg/mL) et la sauge blanche qui contenait des montants minimes (0,003 mg/mL). De plus, la sauge commune et le romarin se sont révélés contenir de l’acide 12-O-méthylcarnosique, avec des concentrations de 1,3 mg/mL et de 0,4 mg/mL respectivement. Ainsi, nous avons démontré que la simple extraction de solvant et la dérivation TMS sont des approches efficaces pour quantifier ces composés bioactifs dans les plantes.

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Publié-e

2026-06-17

Numéro

Vol. 5 No. 1 (2026)

Rubrique

Recherche originale

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