En haut : Le comportement de la surface reflète le remplissage de la vessie natatoire chez le poisson-zèbre

Auteurs-es

  • Iniya Luckshman University of Ottawa, Ottawa, ON, Canada
  • Michael Tea University of Ottawa, Ottawa, ON, Canada
  • Kathleen M. Gilmour University of Ottawa, Ottawa, ON, Canada

DOI :

https://doi.org/10.18192/osurj.v5i2.8054

Résumé

Chez le poisson-zèbre, la vessie natatoire se remplit en avalant de l’air à la surface de l’eau. Ajuster le volume de la vessie natatoire permet au poisson d’atteindre une flottabilité neutre à une profondeur donnée. Les poissons-zèbres dépourvus d’expression fonctionnelle du transporteur de recapture de la sérotonine (5-HT) Sertb (sertb-knockout, sertb-KO ; slc6a4b) présentent un « comportement de remontée », passant pratiquement tout son temps au sommet de la colonne d’eau. Dans la présente étude, nous avons cherché à déterminer si le comportement de remontée à la surface chez les poissons sertb-KO est associé à un remplissage de la vessie natatoire et à la flottabilité. Nous avons mesuré la flottabilité en plaçant des larves de poisson-zèbre Sertb-KO anesthésiées à 7 jours après la fécondation (DPF) dans une colonne d’eau pour évaluer la vitesse et la direction des mouvements, et a calculé le volume de la vessie natatoire à partir de photographies. Les larves sertb-KO présentaient une flottabilité plus élevée que les larves de type sauvage (WT), et une flottabilité plus élevée était associée à un volume de vessie natatoire plus important. Le traitement des larves de WT avec la fluoxétine, un inhibiteur sélectif du Sert, était également associé à une flottabilité et un volume de vessie natatoire plus élevés. Inversement, le traitement des larves de sertb-KO avec l’inhibiteur de la synthèse de la sérotonine p-chlorophénylalanine (pCPA) a entraîné une diminution de la flottabilité et un volume de vessie natatoire plus réduit. De plus, les larves WT traitées avec un agoniste du récepteur 5-HT1A ont montré une flottabilité et un volume de vessie natatoire plus élevés. Ces résultats impliquent une natation élevée en volume de la vessie dans le comportement de sortie et confirment un lien entre des niveaux élevés de sérotonine et le volume de la vessie natatoire, probablement médiés par le récepteur 5-HT1A. Une question clé restante est si le comportement de remontée à la surface est causé par un surremplissage de la vessie natatoire médiée par la sérotonine, ou si les larves de sertb-KO choisissent d’être à la surface et de remplir trop la vessie natatoire pour y rester.

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Publié-e

2026-06-17

Numéro

Vol. 5 No. 2 (2026)

Rubrique

Résumés d’initiation à la recherche en sciences au premier cycle

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