Quantification de la masse tracante en tomographie par émission de positrons cardiaques à l’aide de la chromatographie en phase liquide à haute performance
DOI :
https://doi.org/10.18192/osurj.v5i2.8078Résumé
Le dysfonctionnement du système nerveux sympathique cardiaque est associé à plusieurs maladies cardiovasculaires et peut être évalué de manière non invasive à l’aide de l’imagerie par tomographie par émission de positons (TEP). La méta-fluorobenzylguanidine marquée au fluor-18 ([18F]mFBG) est un radiotraceur qui cible le transporteur de la noradrénaline et permet une évaluation quantitative de la fonction sympathique. Cependant, les formulations du traceur contiennent à la fois des formes radioactives et non radioactives, et un excès de masse non radioactive peut entrer en compétition pour la liaison au transporteur, ce qui peut affecter la précision des mesures d’imagerie. Ce projet vise à développer une méthode basée sur la chromatographie liquide à haute performance (CLHP) afin de quantifier la masse totale du traceur et de déterminer l’activité molaire du [18F]mFBG. Des standards de calibration de mFBG non radioactif seront préparés dans des conditions de solvant correspondant à la formulation du traceur et analysés par CLHP avec détection UV afin de générer une courbe d’étalonnage. Des échantillons de traceur formulé seront ensuite analysés pour déterminer la concentration totale de mFBG, et l’activité molaire sera calculée comme le rapport entre la radioactivité et la masse totale du traceur. Cette approche devrait permettre d’assurer une masse de traceur biologiquement négligeable dans les études précliniques en TEP et pourrait soutenir une future translation clinique.
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